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What programmer u r using..? & what tool u r using..? Please sumup exactly what is the problem..!
The possible reasons could be,
1. Ensure ur make file settings incase of WINAVR.
2. Ensure ur programmer pins since in some case the MOSI, Vcc, Gnd & MISO lines are juggled.
3. Ensure it shows “device connected” in atmel studio.